Наукові статті

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Browsing Наукові статті by Author "KONOVALOVA Olena"

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    Identification of the main compounds of Agastache Foeniculum essential oil by TLC and chemotype express-determination
    (Журнал Фітотерапія. Часопис. Науково-практичне фахове видання, 2023-02-17) KONOVALOVA Olena; HURTOVENKO Iryna; SHURAEVA Tamara
    Topicality. Agastache foeniculum essential oil has bactericidal, fungicidal and anti-inflammatory effects. Depending on the dominant component of the essential oil, five Agastache chemotypes have been distinguished. So, identification of the main compounds of Agastache foeniculum essential oil in other to find markers for the chemotype determination and standardization of raw materials is advisable. The purpose of the research was to study the possibility of identifying the main terpenoids in the Agastache foeniculum raw materials by the method of “cold” thin layer chromatography (TLC) for the chemotype determination. Materials and methods. The TLC was used for investigation with ethyl acetate – toluene (1:19) as mobile phase and special conditions: a temperature of elution +15o C and drying of chromatographic plates in a stream of cool air. Research results. Menthol, linalool and pulegone by the method of “cold” TLC, in the Agastache foeniculum raw material grown in Kyiv were identified. The predominance of pulegone allows us to attribute the raw materials to pulegone chemotype. Estragole and limonene were identified in the Agastache foeniculum raw material grown in Kherson region with a predominance of estragole that allows us to attribute this raw material to the estragole chemotype. Conclusions. The technique above of terpenoids identification ensures the specificity of the determination of the main components of Agastache foeniculum essential oil for the chemotype determination and its standardization.
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    STUDY OF THE MINERAL ELEMENT CONTENT OF RED OAK (QUERCUS RUBRA L.) IN COMPARISON WITH SOIL
    (JOURNAL OF FACULTY OF PHARMACY OF ANKARA UNIVERSITY, 2024-05-20) KONOVALOVA Olena; OMELKOVETS Tetiana; HURTOVETKO Iryna; KALISTA Mariia; SHCHERBAKOVA Olha; SYDORA Natalia
    Objective: The purpose of the present work was to to determine the content of mineral elements in annual shoots with leaves and in the fruits of wild individuals of red oak (Quercus rubra L.) from two different places of growth in comparison with their content in the soil under the plants. Material and Method: The annual shoots with leaves of red oak with soil samples under plants were collected in August 2022 and fruits collected in September 2022 in Ukraine in the mixed forests near Tynne village (Rivne Oblast) and near Lisnyky village (Kyiv Oblast). The research was carried out by the X-ray fluorescence method on the “ElvaX-med” energy dispersive spectrometer. Result and Discussion: As a result of the study, both types of raw materials of Quercus rubra (annual shoots with leaves and fruits) revealed the presence of 4 macro- (S, Cl, K, Ca), 8 micro-(Mn, Fe, Cu, Zn, Br, Rb, Sr, Pb) and 4 ultramicroelements (Cr, Co, Ni, Zr). It was determined that red oak plants are concentrators of potassium and sulfur from the soil (which is indicated by the high content of these macroelements both in the soil under the plants and in all studied raw materials). A high content of calcium in the raw material of red oak from both locations was noted, and this content is apparently characteristic of the plant itself, regardless of the soil on which it grows.